hutP [2017-10-17 13:01:05]

transcriptional antiterminator of the [gene|search|hut ]operon

locus: BSU39340

pI: 6.06

mw: 16.43 kDa

protein length: 151 aa

gene length: 453 bp

function: regulation of histidine utilization

product: transcriptional antiterminator

essential: no

synonyms: hutP1

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.5|Utilization of histidine]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]

Gene

Coordinates: 4,041,483 → 4,041,938

The protein

Protein family

• hutP family (according to Swiss-Prot)

[SW|Cofactors]

• Mg²⁺ [pubmed|29035529]

Effectors of protein activity

• HutP binds its RNA target to cause antitermination in the presence of histidine [Pubmed|23748184]

Structure

• [PDB|3BOY] (complex bound to the [gene|search|hut ]mRNA), [PDB|1WPT]

Expression and Regulation

Operons

• genes: [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]-[gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH]-[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]-[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]-[gene|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG]-[gene|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8071225], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8071225], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|8682780], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|HutP]: antitermination, at a protein-dependent [SW|RNA switch] located between [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP] and [gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH] [Pubmed|8071225], in [regulon|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|HutP regulon]

  regulation

  • induced by histidine ([protein|search|HutP]) [Pubmed|8071225]

Biological materials

Mutant

• BKE39340 (Δ[gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAATCACTCAATTCC, downstream forward: _UP4_TGAAACAGCCCATAGATCTT
• BKK39340 (Δ[gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAATCACTCAATTCC, downstream forward: _UP4_TGAAACAGCCCATAGATCTT

References

Reviews

• 17395359,16427271,22933560

Research papers

• 28510182,23748184,10712704,18445631,15758992,15242603,14763987,15273277,18445631,8071225,16192572,29035529